ligand
2-mercaptopyridine
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5110-01
storage condition
(20% Ethanol)
parameter
<4 mL/min flow rate, 42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-13
working range
3-11
storage temp.
2-8°C
General description
HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.
Application
HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
Features and Benefits
- Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
- Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
- Recovery of IgM is high with excellent activity retention.
- The top and bottom frits are manufactured from porous polyethylene
- Columns are delivered with a stopper on the inlet and a snap-off end on the outle
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids