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GE17-5159-01

HiTrap® Q XL

Cytiva 17-5159-01, pack of 5 × 5 mL

Synonym(s):

IEX column, Q Sepharose XL, chromatography cartridge, cytiva 17-5159-01, ion exchange resin, prepacked column, strong anion exchanger

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151816
Technical Service
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ligand

quaternary amine

description

Ion Exchanger Type (value)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5159-01

parameter

<20 mL/min flow rate, 42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

2-12

suitability

suitable for bioprocess medium

General description

HiTrap® Q XL are strong anion exchangers prepacked with Q Sepharose™ XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Application

Q Sepharose™ XL and SP Sepharose™ XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Features and Benefits

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

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pictograms

Flame
GHS02

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Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

View All Protocols

Articles

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

View All Articles

Global Trade Item Number

SKUGTIN
GE17-5159-0104061837621901