ligand
recombinant protein L (E. coli), mammalian free
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5478-51
parameter
5 bar
column I.D.
7 mm
matrix
rigid, highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
working range
2-10(pH interval where the medium can be operated without significant change in function.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
HiTrap® Protein L columns are packed with Capto™ L affinity chromatography resin. It facilitates the purification of antibody fragments comprising the κ light chain. Capto™ L resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which binds to the variable region of antibody κ light chains with strong affinity. This resin is an effective capture platform for antibody fragments having κ light chains due to its strong binding capacity and affinity to a wide spectrum of antibody fragments.
Application
HiTrap® Protein L may be used to characterize human colorectal cancer MDR1/P-gp Fab and for the purification optimization of a single-chain variable fragment (scFv) against type 1 insulin-like growth factor receptor (IGF-1R). It may also be used for the purification of antigen-binding fragments (Fabs) and domain antibodies (dAbs).
Features and Benefits
- Captures a wide range of antibody fragments
- Allows a platform approach for the purification of antibody fragment
- Offers low ligand leakage, high binding capacity, and selectivity for a broad range of antibody fragments
- Reduces process time and amount of resin
- Suitable for process development and small-scale purification
- HiTrap® format easily connects to a syringe, peristaltic pump, or chromatographic equipment, such as an ÄKTA® system.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
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Warning
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存储类别
3 - Flammable liquids