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About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
Material:
resin (Sephacryl® S-400 HR)
Working volume:
25—100 μL
Sterility:
non-sterile
Packaging:
pack of 50 columns
working range
3-11
material
resin (Sephacryl® S-400 HR)
sterility
non-sterile
packaging
pack of 50 columns
manufacturer/tradename
Cytiva 27-5140-01
storage condition
(20% Ethanol), (TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6))
working volume
25—100 μL
matrix
allyl dextran and N, N’-methylenebisacrylamide
particle size
25-75 μm
avg. part. size
50 μm
cleaning
2-13
suitability
suitable for bioprocess medium
storage temp.
2-8°C
Related Categories
General description
MicroSpin™ columns are designed for the rapid purification of nucleic acids for use in a wide range of applications, including desalting, buffer exchange and removal of primers. Good product yield and purity is obtained with sample volumes from 25-100 μl, and from nanogram to milligram quantities of DNA.
Features and Benefits
- illustra™ MicroSpin™ S-400 HR Columns: For rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography. - Accommodates 25 to 50 μ for post-PCR cleanup prior to cloning or a second amplification reaction or 51 to 100 μ for all other applications.
- Convenient: Prepacked with Sephacryl® S-400 HR pre-equilibriated in TE buffer.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single- and double-stranded exonuclease and RNase assays.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
illustra is a trademark of Cytiva
MicroSpin is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
Storage Class
12 - Non Combustible Liquids
Regulatory Information
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