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Merck
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GE27-9264-01

Ready-to-go You-Prime First-Strand Beads

Cytiva 27-9264-01

Synonym(s):

RTG PCR mix, cDNA template reaction, cocktail, enzymes, polyadenylated RNA assay, total RNA kit

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About This Item

UNSPSC Code:
41116006
NACRES:
NA.55
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feature

hotstart: no

manufacturer/tradename

Cytiva 27-9264-01

color

colorless

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General description

The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 mL tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

Features and Benefits

  • For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice for RT-PCR.
  • Predispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance.
  • First-strand reaction beads contain no primer, thus allowing users to select a first-strand primer of choice.
  • Highly suitable for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples.
  • Reaction beads are function-tested for first-strand synthesis of cDNA up to 7.5 kb and in RT-PCR from blood samples.
  • Completed reactions (33 μl final volume) can be used directly in PCR after adding water, Taq DNA polymerase, and primers; first-strand cDNA can also be used as a template for traditional Gubler-Hoffman second-strand cDNA synthesis.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ready-To-Go is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Regulatory Information

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