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GE28-4009-89

His MultiTrap™ High Performance

Cytiva 28-4009-89, pack of 4 plates

Synonym(s):

High Performance His Trap, His MultiTrap, MultiTrap for His

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About This Item

NACRES:
NA.32
UNSPSC Code:
12161501

Key Documents

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material

polypropylene and polyethylene

form

prepacked column

crosslinking

cross-linked

packaging

pack of 4 plates

manufacturer/tradename

Cytiva 28-4009-89

storage condition

(20% Ethanol)

parameter

-0.5 bar vacuum

technique(s)

liquid chromatography (LC): suitable

size

127.8 mm × 85.5 mm × 30.6 mm

matrix

highly cross-linked 6% agarose

matrix active group

Ni2+ ions functional group

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

separation technique

affinity

Related Categories

General description

His MultiTrap™ FF and His MultiTrap™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).

Application

His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose™ 6 Fast FLow (His MultiTrap™ FF) or Ni Sepharose™ High Performance (His MultiTrap™ HP). A protocol for His MultiTrap™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.

Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.

Features and Benefits

  • Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
  • Increased convenience and consistency with prepacked 96-well plates.
  • No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
  • Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
  • Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.

Preparation Note

Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Articles

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

See All

Protocols

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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