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GE28-4058-46

HiTrap® Capto™ Adhere

Cytiva 28-4058-46, pack of 5 × 5 mL

Synonym(s):

anion, chromatography cartridge, mAB resin, multimodal matrix, polishing media, prepacked column

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About This Item

NACRES:
NA.56
UNSPSC Code:
23151817
Technical Service
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ligand

N-benzyl-N-methyl ethanolamine

description

Ion Exchanger Type (value)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-4058-46

parameter

<20 mL/min flow rate, 42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked agarose

avg. part. size

75 (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-14(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)

working range

3-12(pH interval where the medium can be operated without significant change in function.)

suitability

suitable for bioprocess medium

General description

HiTrap Capto adhere is prepacked with Capto adhere, a multimodal BioProcess ion exchange resin designed to remove contaminats in post-protein A purification. Capto adhere is a strong anion exchanger with multimodal functionality. The multimodal functionality gives a different selectivity compared to traditional anion exchangers. Capto adhere is designed for post-protein A purification of monoclonal antibodies. Removal of leached protein A, aggregates, host cell proteins, nucleic acids, and viruses from monoclonal antibodies is performed in flowthrough mode at which the antibodies pass directly through the column while the contaminants are adsorbed. For optimal performance of Capto adhere, screening/optimization of loading conditions are required.

Application

Protein polishing using Capto™ Adhere Multimodal chromatography

Features and Benefits

  • High capacity and productivity in flowthrough mode.
  • Savings in time and operating costs with a two-step chromatographic process.
  • Wide operational window of pH and conductivity.
  • Prepacked HiTrap columns for easy screening and convenient process development.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva

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pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Global Trade Item Number

SKUGTIN
GE28-4058-4604061837623059