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Merck
CN

GE28-4064-15

Tricorn 10/100 Column

Synonym(s):

chamber, chromatography column, cytiva 28-4064-15, separatio column, tube, unpacked column

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About This Item

NACRES:
SB.52
UNSPSC Code:
41121800
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technique(s)

LPLC: suitable

General description

Tricorn 5 and 10 Coarse Filter Kit is used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Both filter and coarse filter kits include top and bottom adapters, bottom filters, and EPDM O-rings, five of each.
Tricorn columns include a glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock, and filter holder.

Features and Benefits

The modern integrated, snap-on design results in a column that is easy to assembly, operate, and maintain.
Broad range of column dimensions suitable for many different purification needs.
Glass tubes coated with plastic film prevent harm if the column is dropped or operated outside its specification.
Locking ring prevents accidental compression of media bed.
Separate packing connector allows simple column packing for a broad range of media.
Tricorn empty columns are designed for high performance separations on small and medium scale. Pressure stability up to 100 bar and durable column material ensure reliable and reproducible operation as well as compatibility with the majority of the commercial lab media.

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Articles

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

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Protocols

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

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This page covers the use of Sepharose Fast Flow for purification of proteins.

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