GE28-9356-04
HiPrep™ 16/60 Sephacryl® S-400 HR
Cytiva 28-9356-04, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size
Synonym(s):
HiPrep Chromatography Column, Sephacryl S-400 HR
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About This Item
NACRES:
SB.52
UNSPSC Code:
41115700
material
polypropylene (PP)
mol wt
20000-8000000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9356-04
parameter
<0.5 mL/min flow rate, <5 mL sample volume, 0.15 Mpa (22 psi)
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
dextran-acrylamide particle platform, allyl dextran and N, N’-methylenebisacrylamide, polymeric
matrix active group
dextran-acrylamide phase
avg. part. size
50 μm
cleaning
2-13
working range
3-11
separation technique
size exclusion (SEC)
Related Categories
General description
HiPrep™ 16/60 Sephacryl® S-400 HR is a prepacked gel filtration column for preparative separation of biomolecules in the molecular weight range of 20 000 to 8 000 000 with sample volumes up to 5 mL.
Application
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Sephacryl® S-400 is optimized for separating polysaccarides and other macromolecules with extended structures e.g. proteoglycans and liposomes.
gel filtration columns for low to medium pressure ÄKTA systems+AE14
Features and Benefits
- Gel filtration columns for low to medium pressure AKTA systems.
- The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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