ligand
porcine heparin
form
prepacked column
crosslinking
cross-linked
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9365-49
storage condition
(20% ethanol containing 0.05 M sodium acetate)
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation), 2 ml/min - 10 ml/min flow rate, 22 psi
technique(s)
liquid chromatography (LC): suitable
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
matrix active group
heparin
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-12
suitability
suitable for bioprocess medium
separation technique
affinity
General description
HiPrep™ Heparin FF 16/10 is prepacked with Heparin Sepharose™ 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
Application
The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose™ 6 Fast FLow with a chemically optimized linkage.
Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.
Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.
Features and Benefits
- 20 mL column optimized for convenient scale-up purification .
- Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins.
- Simple operation with single pump configurations, as well as ÄKTA® design systems.
- Excellent flow characteristics.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol)
Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
flash_point_f
Not applicable
flash_point_c
Not applicable