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GE28-9440-06

Protein A Mag Sepharose™

Cytiva 28-9440-06, pack of 1 × 500 μL

Synonym(s):

affinity capture resin, cytiva 28-9440-06, magnetic pull-down beads, particles, protein A coupled magnetic beads

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500
Technical Service
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ligand

native protein A

packaging

pack of 1 × 500 μL

manufacturer/tradename

Cytiva 28-9440-06

storage condition

(20% Ethanol, 20% medium slurry)

parameter

Room temperature temp. range

matrix

paramagnetic, spherical, highly cross-linked agarose particles

capacity

8- 17 mg binding capacity(human IgG/ml gel)

storage temp.

2-8°C

General description

Protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose™ with native Protein A as ligand.

Application

Protein A Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with native Protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species.

There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.

Protein A Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

Features and Benefits

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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GHS02

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Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Protocols

Purification of IgG Antibodies with Mag Sepharose®

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

Articles

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Global Trade Item Number

SKUGTIN
GE28-9440-0604061834075158