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Merck
CN

GE28-9505-15

HiScreen™ SP High Performance

Cytiva 28-9505-15

Synonym(s):

AKTA column, IEX resin, cation, chromatography cartridge, ion exchange media, prepacked column

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About This Item

NACRES:
NA.56
UNSPSC Code:
47101511

Key Documents

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Technical Service
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ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9505-15

parameter

44 psi

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

column I.D.

7.7 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

3-14

working range

4-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiScreen™ SP HP columns are prepacked with SP Sepharose™ High Performance ion exchange chromatography media and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX HP columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Features and Benefits

  • SP Sepharose™ High Performance is a strong cation exchange BioProcess medium designed for intermediate and polishing steps in downstream processing.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 2 to 8 °C (20% Ethanol + 0.2 M Sodium Acetate)
Store at 2 to 8°C (20% Ethanol, 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

See All

Protocols

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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