ligand
glutathione
packaging
pkg of 50 ea
manufacturer/tradename
Cytiva 28-9523-59
bed volume
50 μL
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium), ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))
General description
Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.
Features and Benefits
- Preserves protein antigenicity and function.
- Yields up to 500 μg of pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
SpinTrap is a trademark of Cytiva
存储类别
12 - Non Combustible Liquids