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Merck
CN

GE28-9523-59

GST Spintrap™

Cytiva 28-9523-59

Synonym(s):

GST affinity columns, GST-tagged purification columns, chromatography columns, glutathione Stepharose 4B, spin columns

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About This Item

NACRES:
NA.56
UNSPSC Code:
41106500

Key Documents

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Technical Service
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Product Name

GST Spintrap™, Cytiva 28-9523-59

matrix

4% agarose

ligand

glutathione

packaging

pkg of 50 ea

manufacturer/tradename

Cytiva 28-9523-59

bed volume

50 μL

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)
 ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

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Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • Preserves protein antigenicity and function.
  • Yields up to 500 μg of pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

Legal Information

SpinTrap is a trademark of Cytiva

Storage Class

12 - Non Combustible Liquids

Regulatory Information

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Articles

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

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Protocols

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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