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Merck
CN

GE28-9782-44

HiScreen™ Ni Fast Flow

Cytiva 28-9782-44

Synonym(s):

AKTA column, IMAC, affinity column, chromatography cartridge, histidine tagged purification resin, nickle matrix, prepacked column

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About This Item

NACRES:
NA.56
UNSPSC Code:
13111000

Key Documents

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Technical Service
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Product Name

HiScreen™ Ni Fast Flow, Cytiva 28-9782-44

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9782-44

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

column I.D.

7.7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein)

suitability

suitable for bioprocess medium

Related Categories

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

Features and Benefits

  • High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

General description

HiScreen™ columns prepacked with Ni Sepharose™ 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Protocols

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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