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Merck
CN

GE28-9782-45

HiScreen™, DEAE Fast Flow

Cytiva 28-9782-45

Synonym(s):

AKTA column, DEAE sepharose FF, IEX resin, anion, chromatography cartridge, ion exchange media, prepacked column

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About This Item

NACRES:
NA.56
UNSPSC Code:
47101511

Key Documents

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ligand

diethylaminoethyl

description

Ion Exchanger Type (value)

form

prepacked column

crosslinking

cross-linked

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9782-45

storage condition

(20% Ethanol)

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation), 22 psi

technique(s)

liquid chromatography (LC): suitable

bed size

7.7 mm × 100 mm

bed volume

4.7 mL

column I.D.

7.7 mm

matrix

6% cross-linked agarose

matrix active group

N+(C2H5)2H functional group

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

2-12

capacity

binding capacity(~ 110 mg HAS/mL resin)

suitability

suitable for bioprocess medium

separation technique

anion exchange

Related Categories

General description

HiScreen™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Features and Benefits

  • DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Protocols

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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