GE28-9893-34
HiLoad® 26/600 Superdex® 75 pg
Cytiva 28-9893-34, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size
Synonym(s):
Size exclusion chromatography
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About This Item
material
fiberglass end pieces (polypropylene reinforced)
glass column
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range)
Mr 0.5-30 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-34
storage condition
(0.2 M sodium acetate, 20% ethanol)
parameter
<13 mL sample volume
0.3 Mpa max. pressure
0.9-4.4 mL/min flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
320 mL
column L × I.D.
60 cm × 26 mm
matrix
cross-linked agarose
dextran-agarose particle platform
matrix active group
agarose
dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
Related Categories
General description
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Application
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
Preparation Note
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
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Articles
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Protocols
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to refold proteins using gel filtration and products from Cytiva.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
Cytiva的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。
Related Content
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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