ligand
recombinant protein G lacking albumin-binding region
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0485-81
availability
not available in North America
storage condition
(20% Ethanol)
parameter
flow rate, 42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.), 25 mg binding capacity(human IgG/column)
storage temp.
2-8°C
General description
HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits
High binding capacity of 25 mg IgG/ml medium and fast binding kinetics.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiTrap is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids