ligand
glutathione
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0486-09
availability
not available in North America
parameter
42 psi (H2O at 25°C)
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-13
working range
4-13
capacity
>25 mg binding capacity (horse liver GST/ml medium)(H2O at 25°C)
General description
GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Application
Tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC System.
Features and Benefits
- High binding capacity.
- Mild elution conditions preserving protein antigenicity and function.
- Easy one-step purifications of Glutathione S-Transferase (GST) tagged proteins resulting in high purity.
- GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as AKTA design.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
GSTrap is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids