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Merck
CN

GE29401324

Sigma-Aldrich

Strep-Tactin XT Sepharose chromatography resin

pkg of 1 × 10 mL, chromatography resin

Synonym(s):

StrepTrap™ High Performance

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About This Item

UNSPSC Code:
23151817
NACRES:
SB.53

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form

resin

crosslinking

cross-linked

packaging

pkg of 1 × 10 mL

manufacturer/tradename

Cytiva 29401324

storage condition

(20% ethanol )

parameter

≤150 cm/hr flow rate (recommended)
0.3 Mpa max. pressure
300 cm/hr max. flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

cross-linked agarose

matrix active group

Strep-Tactin XT

particle size

~34 µm

operating pH

6—10

capacity

~10 mg binding capacity (protein with Strep-tag II or Twin Step-tag / mL resin)

suitability

suitable for chromatography

separation technique

affinity

storage temp.

2-8°C

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General description

Strep-Tactin XT Sepharose chromatography resin is ideal for the purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilized Strep-Tactin XT ligand giving a highly pure target protein. Strep-Tactin XT Sepharose contains the latest Strep-Tactin ligand, XT, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein than with the predecessor product, StrepTactin Sepharose High Performance (HP). Strep-Tactin XT Sepharose also enables purification under denaturing conditions.

Application

Strep-Tactin XT Sepharose chromatography resin is suitable for the purification of recombinant proteins tagged with a Strep-tag& II and Twin-Strep-tag.

Features and Benefits

  • Ensures highly pure Strep-tag II and Twin-Strep-tag recombinant proteins
  • Fast purifications with a simple elution process
  • Physiological conditions and mild elution preserve target protein activity
  • Fast and easy regeneration of the purification system
  • Compatible with a range of detergents, denaturants, and other additives, allowing flexibility in experimental conditions
  • Stable in commonly used aqueous buffers, ensuring reliable performance across different experiments

Legal Information

StrepTrap is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H225

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C

Regulatory Information

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