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About This Item
NACRES:
NA.31
UNSPSC Code:
41105300
Product Name
ECL™ Direct Nucleic Acid, Cytiva RPN3001, pack of 1 ea
packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN3001
storage temp.
2-8°C
Related Categories
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Features and Benefits
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very Low backgrounds.
General description
ECL™ Direct Labeling and Detection System.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
signalword
Danger
Storage Class
12 - Non Combustible Liquids
Regulatory Information
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Articles
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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