grade
Molecular Biology
manufacturer/tradename
Lucigen 30250-1
packaging
pkg of 50 x 25 µL reactions
technique(s)
RT-PCR: suitable
shipped in
dry ice
storage temp.
−20°C
General description
RapiDxFire Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90 ⁰C) and maintains its core activity after >3 months storage at 20-25⁰C. This enzyme lacks RNAse H and 3’ 5’ exonuclease activity and efficiently synthesizes short cDNA fragments (≤ 1 Kb). RapiDxFire RT possesses DNA polymerase activity but lacks 5′ 3′ exonuclease activity. Content includes RapiDxFire Thermostable Reverse Transcriptase (3U/μl) and 10X RapiDxFire Reaction Buffer.
Application
Reverse Transcriptase, First-strand cDNA synthesis (<1Kb)
Features and Benefits
- Extremely active at high temperatures (55 °C to 80 °C): Improves specificity of cDNA Synthesis from diverse RNA templates
- Sensitive: Detects 100 copies of RNA in two-step RT-qPCR assays
- Short reaction time (5 minutes or less): Streamlined RT-qPCR workflow and faster time to results
- Batch to batch reproducibility: Manufactured in an ISO 13485-certified facility
- Stable at room temperature (> 3 months): Simplifies setup on automation decks (no cold storage required) and enables use in environments with limited cold-storage
- Lyo-compatible: Enzyme formulation is free of glycerol -and other components- that are known to interfere with downstream lyophilisation
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pcodes
Hazard Classifications
Met. Corr. 1
hcodes
signalword
Warning
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable