biological source
Escherichia coli
manufacturer/tradename
Lucigen 60081-1
packaging
pkg of 12 reactions (12 x 25 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 4 x 1010 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α&trade, DH10B&trade, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability while decreasing costs. E.Cloni 10G Supreme Electrocompetent Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.
Packaged as SOLOs (1 transformation per tube, 12 x25µl).
Packaged as SOLOs (1 transformation per tube, 12 x25µl).
Application
Clone, construct libraries, transform Gibson Assembly reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and demanding clonings.
Features and Benefits
- Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
- Supports cloning methylated DNA, BAC and Cosmid Cloning
- Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)
Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA
Components:
E. cloni 10G SUPREME Electrocompetent Cells
pUC 19 Transformation Control
Recovery Media
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable