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Merck
CN

LGC604012

E. cloni EXPRESS BL21(DE3) Chemically Competent Cells (DUOs)

pkg of 24 reactions (12 x 80 µL), Cfu/µg ≥ 1 x 107

Synonym(s):

Competent Cells for protein expression, DNA cloning, DNA transformation, Heat shock transformation, competent E.Coli

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About This Item

UNSPSC Code:
41106202
NACRES:
NB.43
Biological source:
Escherichia coli
Growth mode:
growth or suspension
Manufacturer/tradename:
Lucigen 60401-2

Key Documents

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biological source

Escherichia coli

manufacturer/tradename

Lucigen 60401-2

packaging

pkg of 24 reactions (12 x 80 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: 1×107 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

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General description

E. cloni EXPRESS BL21(DE3) Chemically Competent Cells are ideal for routine protein expression applications. These strain carries a chromosomal copy of the T7 RNA Polymerase gene under the control of the lacUV5 promoter and it is suitable for production of protein from target genes cloned into T7 driven expression vectors.

Application

Cloning, routine protein expression

Features and Benefits

  • Routine protein expression
  • High transformation efficiency
  • Short protein expression protocol time

Transformation efficiency: ≥ 1 x 107 (cfu/µg pUC19)

Genotype: F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Components:


E.Cloni EXPRESS BL21 (DE3) Electrocompetent Cells


pUC 19 Trasformation Control


Recovery Media

Regulatory Information

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