biological source
Escherichia coli
manufacturer/tradename
Lucigen 60512-1
packaging
pkg of 12 reactions (6 x 50 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 4 x 1010 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
SS320 (MC1061 F´) Electrocompetent Cells deliver ≥4 x 1010 cfu/μg of DNA and are particularly useful for phage display protein expression. SS320, also known as MC1061 F´ cells, are also suitable for M13 phage work, general cloning, blue/white screening and protein expression.
Application
Phage Display Libraries, M13 phage display
Features and Benefits
- Perfect for antibody phage display library creation, suitable for M13 phage display
- Create larger libraries; speed recovery
- Supports cloning of methylated DNA, blue-white screening capable
Transformation efficiency: ≥ 4 x 1010 (cfu/µg pUC19)
Genotype: SS320 (MC1061F’): F’[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1
Components:
SS3SS320 (MC1061 F′) Electrocompetent Cells
pUC 19 Trasformation Control
Recovery Media
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable