biological source
Escherichia coli
manufacturer/tradename
Lucigen EC6P095H
packaging
pkg of 10 reactions (5 x 100 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 5×109 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
The TransforMax EC100D pir + Electrocompetent E. coli and TransforMax EC100D pir-116 Electrocompetent E. coli each constitutively express the π protein (the pir gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγ ori). The cells are derived from Epicentre′s TransforMax EC100 Electrocompetent E. coli by P1 phage transduction with a strain containing the pir + or pir-116 gene linked to a dihydrofolate reductase (DHFR) marker.
The TransforMax EC100D pir + cells will maintain R6Kγ ori containing plasmids at approximately 15 copies per cell, 1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell.
Packaged as 5 tubes x 100 µL of electrocompetent cells, which is enough cells for 10 electroporations
The TransforMax EC100D pir + cells will maintain R6Kγ ori containing plasmids at approximately 15 copies per cell, 1 for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell.
Packaged as 5 tubes x 100 µL of electrocompetent cells, which is enough cells for 10 electroporations
Application
Plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits
Features and Benefits
- Rescue cloning of EZ-Tn5 transposed elements
- Propagate plasmids tagged with the EZ-Tn5 Insertion Kit
- Maintains plasmids at ~250 copies per cell.
Genotype TransforMax EC100D pir-116 Electrocompetent E. coli: F - mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str R) nupG pir-116(DHFR)
Components:
TransforMax EC100D pir-116 Electrocompetent E. coli
pR6Kan Control Plasmid
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable