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MIRAP01097

Sigma-Aldrich

MystiCq® microRNA qPCR Assay Primer

hsa-miR-4492

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NACRES:
NA.55

form

liquid

concentration

10 mg/mL

mature sequence

GGGGCUGGGCGCGCGCC

mp

~77 °C

Sanger mature/minor accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

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MIRAP01080MIRAP01049MIRAP01054
concentration

10 mg/mL

concentration

10 mg/mL

concentration

10 mg/mL

concentration

10 mg/mL

mature sequence

GGGGCUGGGCGCGCGCC

mature sequence

UGGCGGCGGUAGUUAUGGGCUU

mature sequence

AAAAGCAUCAGGAAGUACCCA

mature sequence

GAAGAUGGACGUACUUU

mp

~77 °C

mp

~77.5 °C

mp

~76 °C

mp

~75 °C

Sanger mature/minor accession no.

MIMAT0019027

Sanger mature/minor accession no.

MIMAT0018994

Sanger mature/minor accession no.

MIMAT0018935

Sanger mature/minor accession no.

MIMAT0018941

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The MystiCq® microRNA qPCR Assay Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific microRNAs with the MystiCq Universal PCR primer and the MystiCq® microRNA SYBR® Green qPCR ReadyMix on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix kit.

Features and Benefits

  • Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs
  • Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence
  • No self-complementarity or primer dimer artifacts with the Universal PCR Primer
  • Optimized primer Tm designed to match the Universal PCR Primer
  • Universal cycling conditions ensures robust amplification for all assays in profiling experiments
  • Optimized PCR product Tm (75-78° C)

Legal Information

MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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