N4662
Nek6, Active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous glycerol solution
别名:
Gene A-Related Kinase 6, NIMA-Related Kinase 6, Never in Mitosis
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关于此项目
Assay:
≥80% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
buffered aqueous glycerol solution
enzyme activity
≥10 units/mg protein
mol wt
protein apparent mol wt ~58 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NEK6(10783)
Application
Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
Biochem/physiol Actions
Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.
Physical form
Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
Other Notes
One unit will incorporate 1 nanomole of phosphate into myelin basic protein (MBP) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm (32p) ATP
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable