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OGS3466

Sigma-Aldrich

PSF-TEF1-COOH-E-TAG - C-TERMINAL E-TAG TAG YEAST PLASMID

plasmid vector for molecular cloning

Synonym(s):
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

conjugate

E-Tag

form

buffered aqueous solution

mol wt

size 6757 bp

bacteria selection

kanamycin

Origin of replication

2Micron
pUC (500 copies)

Peptide cleavage

no cleavage

Peptide tag location

C-terminal

Promoter

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

reporter gene

none

shipped in

ambient

storage temp.

−20°C

yeast selection

uracil

General description

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.

About the Peptide Tag:This plasmid contains a c-terminal Etag epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: GAPVPYPDPLEPR.

About the Cleavage Tag:This plasmid does not contain a protease cleavage site.

Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.

Sequence

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Other Notes

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Legal Information

Oxford Genetics is a trademark of Oxford Genetics Ltd

Regulatory Information

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Certificate of Analysis

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Certificate of Origin

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