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P2124

Anti-phospho-Polo-Like Kinase Kinase (pSer482, pSer486, pSer490) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-phospho-PLKK

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44
MDL number:
MFCD08705499
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
2

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen ~120 kDa

species reactivity

Xenopus

technique(s)

western blot: 1:1,000 using Xenopus lysates

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Gene Information

Xenopus laevis ... stk10-a(373731)

General description

Polo-Like Kinase Kinase (PLKK) is a 120kD protein that belongs to the serine/threonine kinase family. It has a crucial role in regulating cellular events in cytokinesis and mitosis. Anti-phospho-polo-like kinase kinase (pSer482, pSer486, pSer490) antibody can be used in immunoblotting (diluted 1:1000). Rabbit anti- phospho-polo-like kinase kinase antibody reacts specifically with phospho-polo-like kinase kinase of xenopus.

Immunogen

synthetic phosphopeptide corresponding to amino acids residues surrounding the Xenopus polo-like kinase kinase phosphorylated on serines 482, 486 and 490.

Application

Anti-phospho-polo-like kinase kinase (pSer482, pSer486, pSer490) antibody can be used in western blotting (diluted 1:1000).

Physical form

Solution in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
115K1504

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Y W Qian et al.
Science (New York, N.Y.), 282(5394), 1701-1704 (1998-11-30)
The Xenopus polo-like kinase 1 (Plx1) is essential during mitosis for the activation of Cdc25C, for spindle assembly, and for cyclin B degradation. Polo-like kinases from various organisms are activated by phosphorylation by an unidentified protein kinase. A protein kinase
Xingjie Ma et al.
Aging cell, 17(6), e12831-e12831 (2018-09-15)
Calcium signaling is emerging as a key pathway controlling cellular senescence, a stable cell proliferation arrest playing a fundamental role in pathophysiological conditions, such as embryonic development, wound healing, cancer, and aging. However, how calcium signaling is regulated is still

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