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SAB1404487

Monoclonal Anti-TPMT antibody produced in mouse

clone 1D4, purified immunoglobulin, buffered aqueous solution

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Monoclonal Anti-TPMT antibody produced in mouse, clone 1D4, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1D4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53.06 kDa

species reactivity

human

technique(s)

capture ELISA: suitable
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-5 μg/mL

isotype

IgG1κ

NCBI accession no.

BC005339

UniProt accession no.

P51580

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TPMT(7172)

Application

Monoclonal Anti-TPMT antibody produced in mouse is suitable for capture ELISA, indirect ELISA, indirect immunofluorescence and western blot analysis.

Biochem/physiol Actions

TPMT (Thiopurine S-methyltransferase) mainly mediates different catalytic S-methylation reaction such as 6-mercaptopurine and 6-thioguanine. It also participates in the metabolism of some drugs such as thiopurine drugs, which is largely influenced by TPMT polymorphisms. Deficiency in TPMT gene causes an autosomal recessive disorder, childhood acute lymphoblastic leukemia.

General description

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q. (provided by RefSeq)

Immunogen

TPMT (AAH05339, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK

Physical form

Solution in phosphate buffered saline, pH 7.4

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
06111-1D4

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H L McLeod et al.
Leukemia, 14(4), 567-572 (2000-04-14)
Thiopurine methyltransferase (TPMT) catalyses the S-methylation of thiopurines, including 6-mercaptopurine and 6-thioguanine. TPMT activity exhibits genetic polymorphism, with about 1/300 inheriting TPMT deficiency as an autosomal recessive trait. If treated with standard doses of thiopurines, TPMTdeficient patients accumulate excessive thioguanine
Hyun-Young Kim et al.
Pharmacogenetics and genomics, 25(3), 143-146 (2015-01-08)
Thiopurine S-methyltransferase (TPMT) is a cytoplasmic enzyme involved in the metabolism of thiopurine drugs and its activity is largely influenced by polymorphisms of the TPMT gene. To date, more than 35 TPMT variants are known to be associated with reduced
Hamza Ben Zeglam et al.
The Libyan journal of medicine, 10, 27053-27053 (2015-03-31)
Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme that catalyses the S-methylation of 6-mercaptopurine and azathioprine. Low activity phenotypes are correlated with polymorphism in the TPMT gene. Patients with low or undetectable TMPT activity could develop severe myelosuppression when they are

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