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SAB4200473

Sigma-Aldrich

Anti-EpCAM ICD antibody, Mouse monoclonal

clone 4A7, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-DIAR5, Monoclonal Anti-EGP-2, Monoclonal Anti-EGP314, Monoclonal Anti-EGP40, Monoclonal Anti-ESA, Monoclonal Anti-EpCAM ICD antibody produced in mouse, Monoclonal Anti-HNPCC8, Monoclonal Anti-KS1/4, Monoclonal Anti-KSA, Monoclonal Anti-M4S1, Monoclonal Anti-MIC18, Monoclonal Anti-MK-1, Monoclonal Anti-TACSTD1, Monoclonal Anti-TROP1, Monoclonal Anti-epithelial cell adhesion molecule
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

4A7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~40 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells.
western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.

isotype

IgG1

UniProt accession no.

P16422

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPCAM(4072)
mouse ... Epcam(17075)

General description

Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia.

Immunogen

synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-EpCAM ICD antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Nuclear and cytoplasmic accumulation of Ep-ICD is frequently detected in human epithelial cancers
Ralhan R, et al.
PLoS ONE, 5(11), e14130-e14130 (2010)
WRN conditioned media is sufficient for in vitro propagation of intestinal organoids from large farm and small companion animals
Powell R H and Behnke M S
Biology Open, 6(5), 698-705 (2017)
Nuclear signalling by tumour-associated antigen EpCAM
Maetzel D, et al.
Nature Cell Biology, 11(2), 162-162 (2009)
Epithelial cell adhesion molecule: more than a carcinoma marker and adhesion molecule
Trzpis M, et al.
The American Journal of Pathology, 171(2), 386-395 (2007)
L R Raposo et al.
Veterinary and comparative oncology, 15(3), 952-967 (2016-05-04)
Here we describe the establishment of a new canine mammary tumour (CMT) cell line, FR37-CMT that does not show dependence on female hormonal signaling to induce tumour xenografts in NOD-SCID mice. FR37-CMT cell line has a stellate or fusiform shape

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