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SAB4200639

Monoclonal Anti-DHX9/RHA antibody produced in rat

clone 8E3, purified from hybridoma cell culture

Synonym(s):

Anti-ATP−dependent RNA helicase A, Anti-ATP-dependent RNA helicase A, Anti-DDX9, Anti-DEAD/H (Asp−Glu−Ala−Asp/His) box polypeptide 9, Anti-DEAH (Asp−Glu−Ala−His) box polypeptide 9, Anti-DEAH (Asp-Glu-Ala-His) box helicase 9, Anti-DEAH (Asp-Glu-Ala-His) box polypeptide 9, Anti-DEAH box protein 9, Anti-DHX9, Anti-Ddx9, Anti-HEL-5, Anti-LKP, Anti-NDH2, Anti-NDHII, Anti-Nuclear DNA helicase II (NDHII), Anti-leukophysin, Anti-mHEL-5, Anti-nuclear DNA helicase II, RHA, RNA helicase A

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Technique(s):
immunofluorescence: 5-10 μg/mL using using HeLa cells, immunoblotting: 1-2 μg/mL using using whole extracts of HeLa cells.
Clone:
8E3, monoclonal
Application:
IF
Citations:
2

Key Documents

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isotype

IgG1

technique(s)

immunofluorescence: 5-10 μg/mL using using HeLa cells, immunoblotting: 1-2 μg/mL using using whole extracts of HeLa cells.

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8E3, monoclonal

form

buffered aqueous solution

species reactivity

mouse, hamster, rat, bovine, canine, monkey, human

concentration

~1 mg/mL

UniProt accession no.

Q08211

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

Monkey ... DHX9(715453)
canine ... DHX9(480032)
human ... DHX9(1660)
mouse ... Dhx9(13211)
rat ... Dhx9(304859)

General description

DHX9 (DEAH (Asp-Glu-Ala-His) box helicase 9) also known as RNA Helicase A, nuclear DNA helicase II (NDHII), belongs to the DExD/H family of helicase superfamily 2, that exhibits both RNA and DNA helicase activity in an ATP dependent reaction.

Immunogen

synthetic peptide corresponding to the N-terminal region of mouse DHX9

Application

Monoclonal Anti-DHX9/RHA antibody produced in rat has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

DHX9 (DEAH (Asp-Glu-Ala-His) box helicase 9) is involved in RNA metabolism including RNA secondary structure rearrangement, RNA-protein interactions, RNA turnover, ribosome biogenesis, translation and small RNA metabolism. It participates in the replication cycles of retroviruses and other RNA viruses. DHX9 is implicated in multiple steps of HIV-1 replication, including transcription, export of unspliced viral RNA from the nucleus, translation, particle assembly and reverse transcription.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk

WGK 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
低风险生物材料
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Certificates of Analysis (COA)

Lot/Batch Number
054M4768V

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DExD/H box RNA helicases: multifunctional proteins with important roles in transcriptional regulation
Fuller-Pace FV
Nucleic Acids Research, 34(15), 4206-4215 (2006)
George W Owttrim
RNA biology, 10(1), 96-110 (2012-10-25)
Similar to proteins, RNA molecules must fold into the correct conformation and associate with protein complexes in order to be functional within a cell. RNA helicases rearrange RNA secondary structure and RNA-protein interactions in an ATP-dependent reaction, performing crucial functions

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