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SAB4200641

Monoclonal Anti-Brg1 antibody produced in rat

Name: Monoclonal Anti-Brg1 antibody produced in rat
Brand: SIGMA

clone 3G4, purified from hybridoma cell culture

别名:

Anti-ATP-dependent helicase SMARCA4, Anti-BAF190, Anti-BAF190A, Anti-BRG1, Anti-BRG1-associated factor 190A, Anti-BRM/SWI2-related gene 1, Anti-MRD16, Anti-RTPS2, Anti-SMARCA4, Anti-SNF2, Anti-SNF2-beta, Anti-SNF2-like 4, Anti-SNF2L4, Anti-SNF2LB, Anti-SWI/SNF related, Anti-SWI2, Anti-actin dependent regulator of chromatin, Anti-brahma protein-like 1, Anti-global transcription activator homologous sequence, Anti-hSNF2b, Anti-homeotic gene regulator, Anti-matrix associated, Anti-member 4, Anti-mitotic growth and transcription activator, Anti-nuclear protein GRB1, Anti-protein BRG-1, Anti-protein brahma homolog 1, Anti-subfamily a, Anti-sucrose nonfermenting-like 4, Anti-transcription activator BRG1

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关于此项目

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

3G4, monoclonal

Species reactivity:

mouse, human

Application:

FACS, IF

Citations:

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属性

biological source

mouse

Quality Segment

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3G4, monoclonal

form

buffered aqueous solution

mol wt

~185 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

flow cytometry: 2.5-5 μg/test using HeLa cells, immunoblotting: 0.1-0.2.0 μg/mL using HeLa nuclear extract, immunofluorescence: 10 μg/mL using HeLa cells

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BRG1(6597)
mouse ... Brg1(20586)

说明

General description

Monoclonal Anti-Brg1 (rat IgG1 isotype) is derived from the hybridoma 3G4 produced by the fusion of mouse myeloma cells (SP2) and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human Brm-related gene-1 (Brg1) protein. Brg1 or SWI/SNF (SWItch/Sucrose non-fermentable) related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A, member 4 (SMARCA4) gene with 35 exons, is encoded by the gene mapped to human chromosome 19p13.

Immunogen

a synthetic peptide corresponding to a sequence at the C-terminal region of human Brg1 protein

Application

Monoclonal Anti-Brg1 antibody produced in rat has been used in:

immunoblotting (~185 kDa)
flow cytometry
immunofluorescence

Biochem/physiol Actions

Brm-related gene-1 (Brg1) (or hBrm) protein is the central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, which is involved in transcriptional modulation of hormone-responsive promoters through binding of the complex to various nuclear receptors and its recruitment to gene-specific promoters. In addition, BRG1 has also been shown to act as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. BRG1 participates in the firing of origins and the process of replication elongation. This function of BRG1 also facilitates embryogenesis. In addition, It functions as a tumor suppressor and maintains genome stability and prevent cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c