SAB4200654
Monoclonal Anti-CHD1 antibody produced in rat
clone 2F11H5, purified from hybridoma cell culture
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
2F11H5, monoclonal
Application:
FACS, IF
Citations:
3
biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2F11H5, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
flow cytometry: 5-10 μg/test using using HeLa cells, immunofluorescence: 20 μg/mL using using human HeLa cells.
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CHD1(1105)
Related Categories
General description
Monoclonal Anti-CHD1 (rat IgG2a isotype) is derived from the hybridoma 2F11H5 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide. Chromodomain-helicase-DNA-binding protein 1 (CHD1) is an ATP-dependent chromatin-remodeling factor expressed majorly in cerebellum and basal ganglia. CHD1 gene is mapped to human chromosome 5q15-q21.1. CHD1 comprises two chromodomains.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminal region of human CHD1.
Application
Monoclonal Anti-CHD1 antibody produced in rat may be used in:
- immunofluorescence
- flow cytometry
- immunoblotting
- immunohistochemistry
Biochem/physiol Actions
Chromodomain-helicase-DNA-binding protein 1 (CHD1) interacts with the trimethylated lysine residues of histones and alters access of the transcriptional apparatus to its chromosomal DNA template. CHD1 is required for maintenance of open chromatin and pluripotency in embryonic stem cells. Missense mutation in the CHD1 gene is implicated in the Pilarowski-Bjornsson syndrome as well as in speech delay and facial dismorphism.
Monoclonal Anti-CHD1 recognizes human CHD1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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Missense variants in the chromatin remodeler CHD1 are associated with neurodevelopmental disability
Pilarowski GO, et al.
Journal of medical Genetics, 55(8), 561-566 (2018)
Familial segregation of a 5q15-q21. 2 deletion associated with facial dysmorphism and speech delay
Zepeda-Mendoza
Clinical case reports, 7(6), 1154-1160 (2019)
Lena Ho et al.
Nature, 463(7280), 474-484 (2010-01-30)
New methods for the genome-wide analysis of chromatin are providing insight into its roles in development and their underlying mechanisms. Current studies indicate that chromatin is dynamic, with its structure and its histone modifications undergoing global changes during transitions in
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