Anti-Calponin antibody, Mouse monoclonal

Anti-Calponin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hCP hybridoma (also cites as CALP), produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Calponin (CNN) exists in three isoforms CNN1, CNN2 and CNN3. CNN2 gene is mapped to human chromosome 19p13.3. All three isoforms are expressed in smooth muscle. While CNN1 expression is confined only to smooth muscle, CNN2 and CNN3 are expressed in other cells including the neuronal tissue, fibroblast, myoblast and B lymphocyte. The three isoforms differ in the C-terminal region and comprise conserved actin-binding middle domain and a N-terminal calponin homology (CH) domain.

human uterus smooth muscle extract

Anti-Calponin antibody, Mouse monoclonal may be used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry

Calponin is an F-actin, calmodulin, and tropomyosin binding protein that presents in both the cytoskeleton and contractile machinery of smooth muscle (SM) cells. The interaction of Calponin with actin is shown to inhibit myosin Mg-ATPase activity. The binding strength of calponin to tropomyosin is weakened in the presence of Ca²⁺. Calponin is involved in a variety of cellular processes including smooth muscle contraction, cell movement, shape change, angiogenesis, exocytosis and endocytosis. Monoclonal anti-Calponin can be used for the identification and localization of calponin in SM elements. Calponin levels may serve as a prognostic factor in hepatocellular carcinoma, renal cell carcinoma, osteosarcoma and melanoma.

Monoclonal Anti-Calponin specifically recognizes smooth muscle Calponin.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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