SAB4200775
Anti-Glycoprotein (MDR) antibody, Mouse monoclonal
clone F4, purified from hybridoma cell culture
Synonym(s):
ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Product Name
Anti-Glycoprotein (MDR) antibody, Mouse monoclonal, clone F4, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
F4, monoclonal
form
buffered aqueous solution
species reactivity
hamster, human
concentration
~1 mg/mL
technique(s)
flow cytometry: suitable
immunoblotting: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ABCB1(5243)
Related Categories
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Application
Anti-Glycoprotein (MDR) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemistry
- cellular enzyme-linked immunosorbent assay (ELISA)
- flow cytometry (FACS)
- cell surface radioimmunoassay (RIA)
Biochem/physiol Actions
Anti-P-Glycoprotein (MDR) antibody, Mouse monoclonal recognizes an epitope located at the N-terminal region of P-glycoprotein (Pgp), at the third extracellular loop of the molecule, also known as ABCB1 protein. The antibody specifically detects MDR1 P-glycoprotein, but does not recognize MDR3. Anti-P-Glycoprotein (MDR) cross reacts with MDR1 P-glycoprotein from human and hamster origin.
Multidrug resistance protein 1 (MDR1) functions as an adenosine triphosphate (ATP)-dependent efflux pump. It is also involved in extruding certain xenobiotics and protect cells from toxicants. MDR1 gene is overexpressed in different cancer types including leukemias, lymphomas, myelomas, breast cancer, ovarian cancer, gastrointestinal stromal tumor (GIST), non-small cell lung cancer (NSCLC), fallopian tube, colon, renal, and thyroid cancers. Overexpression of MDR1 gene plays an important role in prompting multidrug resistance in cancer chemotherapy causing high resistance of cancerous cells to a wide variety of substrate anticancer drugs.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-P-Glycoprotein (MDR) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma F4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Multidrug resistance protein 1 (MDR1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporters superfamily. It is ubiquitously expressed in kidneys, intestines, placenta, liver, adrenal glands blood-brain barrier (BBB), placenta and testis.
Immunogen
a mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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MDR1 genotype-related pharmacokinetics and pharmacodynamics
Sakaeda T, et al.
Biological & Pharmaceutical Bulletin, 25(11), 1391-1400 (2002)
Multidrug resistance in breast cancer: a meta-analysis of MDR1/gp170 expression and its possible functional significance
Trock B J, et al.
Journal of the National Cancer Institute, 89(13), 917-931 (1997)
Xing-Duo Dong et al.
Frontiers in pharmacology, 13, 843829-843829 (2022-03-15)
The KRAS-G12C inhibitor ARS-1620, is a novel specific covalent inhibitor of KRAS-G12C, possessing a strong targeting inhibitory effect on KRAS-G12C mutant tumors. Overexpression of ATP-binding cassette super-family B member 1 (ABCB1/P-gp) is one of the pivotal factors contributing to multidrug
Overexpression of ABCB1 Transporter Confers Resistance to mTOR Inhibitor WYE-354 in Cancer Cells
Wang J, et al.
International Journal of Molecular Sciences, 21(4), 1387-1387 (2020)
ATP hydrolysis-dependent multidrug efflux transporter: MDR1/P-glycoprotein.
Kimura Y, et al.
Current Drug Metabolism, 5(1), 1-1 (2004)
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