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Merck
CN

SAB4702031

Monoclonal Anti-PSMA purified produced in mouse

Synonym(s):

FGCP, FGGCP, FOLH1, GCP2, NAALADase I, PGGCP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
GCP-04, monoclonal
Application:
ICC, IHC, WB
Citations:
-
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conjugate

unconjugated

antibody product type

primary antibodies

clone

GCP-04, monoclonal

form

buffered aqueous solution

species reactivity

( Rat), ( Mouse), ( Human), pig

concentration

1 mg/ml

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

storage temp.

2-8°C

Quality Level

Gene Information

human ... LOLH1(2346)

General description

Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.

Immunogen

Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells

Biochem/physiol Actions

The mouse monoclonal antibody GCP-04 recognizes amino acids 100-104 of extracellular domain of denaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately 95-110 kDa transmembrane glycoprotein.

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified by protein-A affinity chromatography.
Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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