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Merck
CN

SAB5900005

Alpaca Anti-mCherry single domain antibody , agarose beads

recombinant Alpaca(VHH) Nano antibody agarose bead suspension

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About This Item

NACRES:
NA.41
Conjugate:
agarose conjugate
Clone:
recombinant monoclonal
Application:
ChIP, IP, RIP, co-IP, mass spectrometry (MS)
Citations:
-

Key Documents

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biological source

alpaca

recombinant

expressed in Pichia pastoris

conjugate

agarose conjugate

antibody product type

primary antibodies

clone

recombinant monoclonal

form

liquid

technique(s)

ChIP: suitable, RIP: suitable, co-immunoprecipitation (co-IP): suitable, immunoprecipitation (IP): suitable, mass spectrometry (MS): suitable

color

colorless

matrix

(~45 um (cross-linked to 7.5% agarose beads))

isotype

VHH

capacity

binding capacity(10 μL slurry binds about 3ug-4μg of recombinant mCherry.)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

General description

Fluorescent protein mCherry is frequently used as reporter and fusion tag. Anti-mCherry single domain antibody , agarose beads are agarose beads covalently coupled with VHH antibodies acquiring high specificity and affinity for mCherry. These beads can efficiently capture and separate mCherry and mCherry tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.

Immunogen

mCherry Tagged Protein

Biochem/physiol Actions

Selectively recognizes mCherry, mRFP, mKate2, mRFPruby, tagRFP, mPlum,and many more RFP derivatives.

Features and Benefits

  • Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
  • They are directly coupled to beads covalently.
  • They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
  • No heavy and light chains in downstream interactions.
  • Acids and alkali resistance, tolerance to harsh conditions.
  • Short incubation time 5 – 30 minutes.
  • Low batch to batch variations.
  • Typically, antibodies are not detectable in Western Blot.

Packaging

plastic tube

Physical form

1XPBS, 0.03% sodium azide, 50% glycerol.

Other Notes

Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.

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Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
20260102
20251102
20221202

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