SRE0117
Salt Active Nuclease (SAN)
High Purity, recombinant, expressed in Pichia pastoris, Triton-free
Synonym(s):
endonuclease, salt active endonuclease, salt tolerant nuclease
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Recombinant:
expressed in Pichia pastoris
recombinant
expressed in Pichia pastoris
composition
Triton-free
packaging
pkg of 25 kU
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
This Salt Active Nuclease (SAN) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
Application
- Useful for purification of tagged recombinant proteins
- Manufacturing of nucleic acid-free molecular enzymes and reagents
- Research of vaccines, viruses and antibodies
Features and Benefits
- Active at low temperatures
- Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
- Typically does not bind to anion exchange or IMAC media
- Detergent free No protease detected
Preparation Note
In solution
Other Notes
- Temperature: 5 – 40°C, optimal: ~35°C
- Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
- Mg2+: >1 mM is required for activity, optimal 5 - 50 mM
- pH: 7.3 – 10.0, optimal 8.2 - 9.2
One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCI pH 8.5 (@25 °C), 5 mM MgCl2, 500 mM NaCl, and 50 µg/mL calf thymus DNA.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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