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SRE0118

Sigma-Aldrich

dsDNase

recombinant, expressed in Pichia pastoris, heat-labile, Triton-free

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Synonym(s):
double strand specific deoxyribonuclease

recombinant

expressed in Pichia pastoris

Quality Level

200

specific activity

(Ca. 400 000 Kunitz Units/mg)

feature

heat-labile

composition

Triton-free

packaging

pkg of 250 U

optimum pH

7.5

shipped in

dry ice

storage temp.

−20°C

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General description

dsDNase is a heat-labile endonuclease that has a strong preference for double-stranded DNA. dsDNase can be heat inactivated by heat treatment at 15 min at 65˚C, or 20 min at 60˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.

Application

This dsDNase is a useful tool to remove contaminating DNA from PCR mastermixes.

Features and Benefits

  • This nuclease has double-stand specificity, which allows specific degradation of dsDNA while leaving shorter ssDNA as primers.
  • Easy inactivation by moderate heat (65°C) allows addition of DNA intended for analysis directly after removal of contaminating DNA.
  • The specific activity is estimated to be 30 times higher than that of bovine DNase I.
Activity towards dsDNA is 5000-fold higher than towards ssDNA

Unit Definition

One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.

Reconstitution

In solution.

Other Notes

The dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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