SRE0118
dsDNase
recombinant, expressed in Pichia pastoris, heat-labile, Triton-free
Synonym(s):
double strand specific deoxyribonuclease
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
(Ca. 400 000 Kunitz Units/mg)
Recombinant:
expressed in Pichia pastoris
recombinant
expressed in Pichia pastoris
specific activity
(Ca. 400 000 Kunitz Units/mg)
feature
heat-labile
composition
Triton-free
packaging
pkg of 250 U
optimum pH
7.5
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
dsDNase is a heat-labile endonuclease that has a strong preference for double-stranded DNA. dsDNase can be heat inactivated by heat treatment at 15 min at 65˚C, or 20 min at 60˚C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.
Application
This dsDNase is a useful tool to remove contaminating DNA from PCR mastermixes.
Features and Benefits
- This nuclease has double-stand specificity, which allows specific degradation of dsDNA while leaving shorter ssDNA as primers.
- Easy inactivation by moderate heat (65°C) allows addition of DNA intended for analysis directly after removal of contaminating DNA.
- The specific activity is estimated to be 30 times higher than that of bovine DNase I.
Preparation Note
In solution.
Other Notes
One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.
The dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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