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SRP0101

Sigma-Aldrich

HDAC 1 from Plasmodium falciparum

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

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Synonym(s):
HD1, Histone deacetylase 1
NACRES:
NA.32

biological source

Plasmodium falciparum

recombinant

expressed in baculovirus infected insect cells

Assay

≥40% (SDS-PAGE)

form

aqueous solution

mol wt

55 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

XM_001352091

UniProt accession no.

Q9XYC7

shipped in

dry ice

storage temp.

−70°C

Gene Information

Plasmodium falciparum ... HDAC1(813532)

General description

Plasmodium falciparum HDAC1, GenBank Accession No. XM_001352091, full length with C-terminal HIS6-DDDDK (FLAG) tags, MW = 55 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 50 mM Tris-HCl, pH 8.0, 130 mM NaCl and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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