SRP0135
G9a human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym(s):
EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
74.6 kDa
biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
74.6 kDa
packaging
pkg of 50 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EHMT2(10919)
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General description
Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3. Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis. G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor. It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy. G9a serves as a target for cancer therapy including breast cancer.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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H3K27me1 is essential for MMP-9-dependent H3N-terminal tail proteolysis during osteoclastogenesis
Kim K, et al.
Epigenetics & Chromatin, 11(1), 23-23 (2018)
G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis
Casciello F, et al.
Proceedings of the National Academy of Sciences of the USA, 114(27), 7077-7082 (2017)
G9a coordinates with the RPA complex to promote DNA damage repair and cell survival
Yang Q, et al.
Proceedings of the National Academy of Sciences of the USA, 114(30), E6054-E6063 (2017)
G9a, a multipotent regulator of gene expression
Shankar S, et al.
Epigenetics, 8(1), 16-22 (2013)
SATB2 suppresses non-small cell lung cancer invasiveness by G9a
Ma Yi-nan, et al.
Clinical and Experimental Medicine, 18(1), 37-44 (2018)
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