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SRP0220

Sigma-Aldrich

DEP1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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Synonym(s):
CD148, DEP-1, Density-enhanced phosphatase 1, PTPRJ, Protein tyrosine phosphatase, receptor type, J
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

aqueous solution

potency

≥0.114 units/mg

mol wt

58.9 kDa

packaging

pkg of 20 μg

concentration

>0.02 mg/mL

NCBI accession no.

NM_U10886

UniProt accession no.

Q12913

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPRJ(5795)

General description

Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Regulatory Information

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James R Whiteford et al.
Molecular biology of the cell, 22(19), 3609-3624 (2011-08-05)
Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the

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