SRP0223
RPTPg Active human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym(s):
PTPRG, Protein tyrosine phosphatase, receptor type, G, R-PTP-GAMMA, Receptor-type protein phosphatase gamma
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Product Name
RPTPg Active human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
aqueous solution
mol wt
65.6 kDa
packaging
pkg of 20 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPRG(5793)
Application
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
General description
Protein tyrosine phosphatase. Catalytic domain, (amino acids 801-1147), (Gene bank accession number NM_002841) with N-terminal GST tag, MW = 65.6 kDa, expressed in an E. coli expression system.
Other Notes
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
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