SRP0224
PTPsigma/PTPRS Active human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym(s):
PTPRS, Protein tyrosine phosphatase, receptor type, S, R-PTP-σ
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352200
Product Name
PTPsigma/PTPRS Active human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
98 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPRS(5802)
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Protein tyrosine phosphatase, receptor type S (PTPRS)/ PTPs localized on the cell surface plays a vital role in cell–cell and cell– extracellular matrix (ECM) interactions, and is implicated in the regulation of axon homeostasis and neuronal development. In addition, PTPRS also has a crucial role in autophagy regulation. PTPRS expressed on plasmacytoid dendritic cells (pDCs) inhibits the production of type I interferon (IFN) in response to viruses. Mutation of both Ptprs and Ptprf (protein tyrosine phosphatase, receptor type F) results in pDC hyperactivation and mild colitis.
General description
Protein tyrosine phosphatase, receptor type S (PTPRS) also known as PTPσ belongs to the subfamily of receptor-type protein tyrosine phosphatases. It is encoded by the gene mapped to human chromosome 19p13.3. PTPRS consists of large ectodomain and a membrane-spanning intracellular domain.
Human PTPRS (GenBank Accession No. BC104812) (amino acids 883-end), with N-terminal GST tag, MW = 98kDa, expressed in a Escherichia coli expression system.
Human PTPRS (GenBank Accession No. BC104812) (amino acids 883-end), with N-terminal GST tag, MW = 98kDa, expressed in a Escherichia coli expression system.
Other Notes
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
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Identification of PTPsigma as an autophagic phosphatase.
Martin KR
Journal of Cell Science, 124(Pt 5), 812-819 (2011)
Physical mapping of receptor type protein tyrosine phosphatase sigma (PTPRS) to human chromosome 19p13.3.
Wagner J
Genomics, 38(1), 76-78 (1996)
Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells.
Bunin A
Immunity, 43(2), 277-288 (2015)
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