SRP0227
SMURF1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
Synonym(s):
E3 ubiquitin ligase SMURF1, KIAA1625, SMAD specific E3 ubiquitin protein ligase 1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
≥0.0055 pmol/min-μg
Assay:
≥80% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL
Product Name
SMURF1 Active human, recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
specific activity
≥0.0055 pmol/min-μg
mol wt
70 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SMURF1(57154)
Application
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Human SMURF 1 (GenBank Accession No. NM_020429), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Other Notes
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
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