biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
75 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... BRSK2(9024)
General description
Human recombinant BRSK2 (GenBank Accession No. NM_003957), full length with N-terminal His tag, MW = 75 kDa, expressed in expressed in baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.