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SRP0279

UBA6 (UBE1L2) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym(s):

MOP-4, UBE1L2, Ubiquitin-activating enzyme E1-like 2, Ubiquitin-like modifier activating enzyme 6

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Assay:
≥70% (SDS-PAGE)
Form:
aqueous solution
Mol wt:
118 kDa
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biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

118 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBA6(55236)

General description

Human UBA6 (UBE1L2) (GenBank Accession No. NM_018227), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.


Regulatory Information

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Global Trade Item Number

SKUGTIN
SRP0279-100UG04061837139048