SRP0415
PKM1 active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
biological source
human
recombinant
expressed in E. coli
Assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
59 kDa
packaging
pkg of 20 μg
manufacturer/tradename
Sigma-Aldrich
storage condition
avoid repeated freeze/thaw cycles
concentration
1.8 mg/mL
technique(s)
cell based assay: suitable
solubility
water: soluble
NCBI accession no.
UniProt accession no.
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PKM(5315)
General description
Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.
Application
- cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
- oxidative stress experiments on PKM2.
- Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).
Biochem/physiol Actions
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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