SRP0724
ERK3 (MAPK6), Active
PRECISIO® Kinase, recombinant
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
frozen liquid
Assay:
70-95% (SDS-PAGE)
Recombinant:
expressed in baculovirus infected Sf9 cells
Mol wt:
~135 kDa
recombinant
expressed in baculovirus infected Sf9 cells
assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
20-30 nmol/min·mg (radiometric)
mol wt
~135 kDa
concentration
0.05 μg/μL
accession no.
NM_002748
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... ERK3(5597)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human ERK3 (MAPK6) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. ERK3 (MAPK6) or mitogen-activated protein kinase 6 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases) which also known as extracellular signal-regulated kinases (ERKs) that are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals and tyrosine phosphorylated in response to insulin and NGF (1). ERK3 is highly expressed in various human tissues, most abundantly in skeletal muscle (2).
Preparation Note
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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